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How it works

ORIGAMI-MS works by interfacing MassLynx and Waters Research Enabled Software (WREnS) to carry out a typically tedious task of increasing the collision or cone voltage prior to ion mobility separation.

The program works by executing a pre-compiled C# code alongside normal acquisition, however the C# (WREnS code) modifies some of the DC potentials on the instrument, taking control away from MassLynx. In ORIGAMI-MS case, we only control three DC potentials, the DRE lens which controls the attenuation of the ion beam, the sample cone voltage (SAMPLE_CONE_VOLTAGE_SETTING) and trap collision voltage (SOURCE_BIAS_SETTING). These are modified on-the-fly, as the acquisition continues, to reflect the user settings. The latter two can be controlled in voltage range of 0 to 200 V, which provides sufficient activation energy to unfold and fragment most compounds.

Note

Some instruments have been modified to have wider activation range. If this is you, please let me know and I can modify the maximum voltage value.